DNA Extraction and Purification
New England Biolabs, Inc. Monarch® Spin PCR & DNA Cleanup Kit - 50 preps
The Monarch Spin PCR & DNA Cleanup Kit (5 ug), a newer version of our original PCR cleanup kit, offers an efficient method for purification and concentration of up to 5 ug of high-quality, double-stranded and single-stranded DNA from enzymatic reactions such as PCR, restriction digestion, ligation, and reverse transcription. This new version features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 5 ul, for highly concentrated DNA in downstream applications such as sequencing, restriction digestion, library construction, labeling and other enzymatic manipulations. Our optimized buffer system allows efficient binding and purification of the DNA, without a need to monitor pH. These kits contain uniquely designed columns and bottles to significantly reduce plastic usage compared to leading suppliers, while maintaining structural integrity for centrifugation, vacuum manifolds and other steps
Epigentek Inc Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) (96 assays)
The Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases.
Boca Scientific Inc Buccalyse Rapid Buccal Cell DNA Isolation Kit, 50 Extractions, with 50 SK-1S Buccal Swabs
The BEK-50/SK1 kit includes 50 SK-1S Buccal Swabs and the Buccalyse DNA extraction kit for 50 samples. SK-1S Buccal Swabs are designed for high-yield DNA retrieval and convenient transport. Each SK-1S buccal swab is packaged with a 5ml plastic collection tube and cap. The Buccalyse DNA Extraction Kit, specifically designed for use with buccal swabs, includes one solution for a fast and simple one tube method for extracting DNA from buccal swabs. In only 20 minutes, this kit provides PCR-ready DNA that does not require centrifugation.
Integrated DNA Technologies Inc Alt-R™ HDR Enhancer V2, Increases HDR Efficiency, Lowers Cytotoxicity, 150 µL
The Alt-R™ HDR Enhancer V2 significantly boosts homology-directed repair (HDR) efficiency in genome editing workflows, providing improved cell viability with lower cytotoxicity compared to previous versions. Its versatility allows use with various delivery methods like electroporation and lipofection, making it ideal for multiple cultured human cell lines. This product uniquely combines effectiveness across diverse applications while minimizing off-target effects.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Orange, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Rat Normal Tissue: Skeletal Muscle, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Cayman Chemical Fungal Lysergol 50mg
An ergot alkaloid and 5-HT1A, 5-HT2A, and 5-HT2C receptor agonist (EC50s = 73, 1.6, and 6.6 nM, respectively, in a FLIPR assay); induces contractions in isolated rat tail arteries (EC50 = 436.5 nM); enhances the antibacterial activity of nalidixic acid against susceptible and drug-resistant E. coli at 10 UG/ml
Biochain Institute Inc Control Genomic DNA - Cynomolgus Monkey Female, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Cerebellum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Research Products International Corp Quick-DNA Plant/Seed Miniprep Kit, 50 Preps
The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology with Zymo-Spin IV-IR HRC filter. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.
Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
Ultra-high density BashingBeads are fracture resistant and chemically inert.
Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Amsbio LLC MagSi-DNA mf
MagSi-DNA mf is ideal for developing your own application, such as: - Isolation of genomic, mitochondrial, or viral DNA from whole blood, cell lysates, human, animal, or plant tissue; isolation of RNA - Isolation of genomic, plasmid, or phage DNA from bacterial cultures and bacteria from clinical samples (blood, stool, swabs, etc.) - Clean-up of DNA from enzymatic reactions or chromatin immunoprecipitation (ChIP) | MagSi-DNA mf consists of magnetic silica beads with a highly dense core of magnetite. Due to their ferrimagnetic properties, the beads typically collect within 10 seconds in a magnetic field, but quickly loose magnetic remanence and are easily resuspended. The beads have minimal adhesion effects to plastics, making them perfectly suitable for use in microfluidic systems. The small particle size offers a very large active surface area for binding of nucleic acids, and the storage buffer has been optimized for maximum suspension time and ease of resuspension.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Brain, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Enzo Life Sciences AMPIPROBE Bacterial Vaginosis Extraction Controls (500µl)
The AMPIPROBE Candida Extraction Controls are designed to be used during the genomic DNA extraction of Atopobium vaginae, Gardnerella vaginalis, Lactobacillus spp., Megasphaera spp., and BVAB2. All performance specifications must be validated by end-user and will vary based on chosen extraction methods. Eluent from extraction can be used as extraction control for AMPIPROBE Bacterial Vaginosis Assay kit (ENZ-GEN205). Plasmids in Extraction Controls are present at higher concentrations compared to assay controls included in the qPCR kit. Extra care should be taken to avoid contamination. Applications: qPCR. Species reactivity: BVAB-2, Megasphaera-1, Lactobacillus spp., Gardnerella vaginalis, Atopobium vaginae.
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), OdT & Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.