DNA Extraction and Purification
Zymo Research Corporation Select-a-Size DNA MagBead Kit
This simple workflow allows for specific cutoffs thatcan be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The Select-a-Size MagBead Buffer selectively binds fragments based on the volume added relative to the sample. Simply choose a desired cutoff to bind the target of interest onto the beads and remove species outside of this range. The desired DNA is easily eluted from the beads following a rapid wash regimen.
Omega Biotek Inc EZNA DNA KIT 200 PREP
The E.Z.N.A.� Tissue DNA Kit offers a simple, rapid, and cost-effective method for the isolation of DNA from a wide variety of sample sources include fresh or frozen animal cells and tissues. After cell lysis, the DNA purification process can be completed in fewer than 30 minutes. Up to 30 mg of tissue at a time can be easily processed using the simple E.Z.N.A.� Tissue DNA protocol. With the spin-column based kit, multiple samples can be processed in parallel. There is no need for phenol/chloroform extractions or time-consuming steps such as precipitation with isopropanol or ethanol. DNA purified using the E.Z.N.A.� Tissue DNA Kit is ready for most downstream applications such as PCR, Southern blots, and restriction enzyme digestion.
New England Biolabs, Inc. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® – 24 reactions
The Ultra II Directional RNA Library Prep Kit for Illumina delivers significantly increased sensitivity and specificity from your RNA-seq experiments, from ever-decreasing amounts of input RNA. In conjunction with ribosomal RNA (rRNA) depletion or poly(A) enrichment, the kit enables the production of high quality libraries from 5 ng or 10 ng of Total RNA, respectively, up to 1 µg. The NEBNext Ultra II Directional RNA Library Prep Kit derives its directionality from the “dUTP” method for strand-specificity, with proven superiority for this application.
- Generate the highest yields of high quality libraries, with a broad range of input amounts
- Increase the complexity and transcript coverage of your libraries
- Optimize your time with streamlined workflows, reduced hands-on time, and automation compatibility
- Rely on robust performance, even with low quality RNA, including FFPE
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Active Motif UDP-Azide-Glucose
UDP-Azide-Glucose is used in conjunction with Beta-glucosyltransferase enzyme (Catalog No. 55012) to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation for analysis. For more complete information, please visit the Active Motif website.
Macherey-Nagel NucleoSpin 96 Blood QuickPure, Efficient DNA Purification, Silica-Membrane Technology, 96-Well Format
The NucleoSpin 96 Blood QuickPure kit offers an efficient solution for rapid DNA purification from various blood samples, utilizing advanced silica-membrane technology. This kit minimizes hands-on time while ensuring high-purity DNA suitable for sensitive applications like PCR. With a capacity of 384 preps, it is designed for streamlined processes and high yields, setting it apart from other products.
New England Biolabs, Inc. NEBNext Ultra™ II DNA Library Prep with Sample Purification Beads – 96 reactions
The NEBNext Ultra II DNA Library Prep with Sample Purification Beads contains the enzymes, buffers and purification beads required to convert a broad range of DNA input amounts into indexed libraries for next-generation sequencing on the Illumina platform. The fast, user-friendly workflow has minimal hands-on time and, for added convenience, this kit contains NEBNext Sample Purification Beads (SPRIselect beads from Beckman Coulter) for size selection and enzyme reaction cleanup.
New England Biolabs, Inc. Monarch® Spin gDNA Extraction Kit - 150 preps
The Monarch Spin gDNA Extraction Kit is a comprehensive solution for cell lysis, RNA removal, and purification of intact genomic DNA (gDNA) from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, bacteria and yeast can be processed with extra steps to enhance lysis in these tough-to-lyse samples. Monarch products use significantly less plastic in columns and kits compared to leading suppliers. Protocols are also included to enable purification from clinically-relevant samples such as saliva and cheek swabs as well as rapid cleanup of previously extracted gDNA. Purified gDNA has high quality metrics, including A260/A280 > 1.8 and A260/A230 > 2.0, high DIN scores and minimal residual RNA. The purified gDNA is suitable for downstream applications such as end-point PCR, qPCR and library prep for NGS. It typically has a peak size of > 50kb, making this kit an excellent choice upstream of long-read sequencing platforms